Biology Biotechnology Principles and Processes Cloning Vectors and Competent Host Single Correct MCQ
Published on: August 13, 2026

In Ti-plasmid, which of the following is removed?

A
Auxin gene
B
Virulent gene
C
Cytokinin gene
D
Auxin and cytokinin gene

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Text Solution

Verified by Experts
The correct answer is:
B

The Ti (Tumor-inducing) plasmid of Agrobacterium tumefaciens contains genes for the synthesis of auxin and cytokinin, which cause tumor formation in plants.

To use the Ti plasmid as a cloning vector, the auxin and cytokinin genes are removed (disarmed) and replaced with the desired foreign gene.

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